#DNA Barcoding
- DNA
- Gene
- DNA Barcode
- DNA Extraction
- Genome
- PCR
- Sampling
- Metadata
- DNA is information
- Metadata makes Data valuable
- Biochemistry allows to examine the cell
- Science is limited (it can only tell us so much)
Over the next several sessions, we will be introducing DNA Barcoding as our prototypical laboratory. By going in-depth we will learn a lot about scientific process as it pertains to working in a molecular biology laboratory.
DNA Barcoding (See the Laboratory Manual)allows us to get a tentative identification of a sample (tissue, food, etc.) based on a unique DNA-Sequence. There are millions or billions of nucleotides in the genome of a eukaryotic organism. Finding the correct sequence for a barcode is not trivial (See: DNA Barcode for Land Plants). In this lab we will begin to understand how to:
- Get DNA from a sample (for example a leaf)
- Localize and copy a specific section of DNA (PCR)
- Read and analyze DNA sequence
PIPETTE DEMO What is a Pipette? easy way to transfer liquid Adjustable pipettes: Works with microliter volumes. Reusable without cross contamination. use disposable plastic tips Highly accurate and precise. Comes in three colors: Blue- 100-1000 Yellow- 10-100 Grey- 0.5-10 Always use the pipette with the smallest range! Remember to match tubes with the correct pipette. blue- blue tips yellow- 200 tips grey- 10 tips Remember to pipette from the first stop.
This laboratory will take several sessions, and we will focus a great deal on good laboratory technique and process (including documentation of metadata)