Issue with demultiplexing Thermo Raw staggered windows

[ZeshanAlimq]

Thanks for building this amazing platform.

I’m attempting to build a chromatogram library using gas-phase fractionation (GPF). I ran six DIA staggered GPFs covering the 400-1000 m/z range on the Thermo Eclipse.
I attempted to demultiplex using MSConvert on Windows using the settings shown in the image.

I'm getting the following error.

Raw files and method files are available here (file://SCI-6682/Users/45774862/OneDrive%20-%20Macquarie%20University/BrainRAW)
Thank you

[nickshulman]

The link where you say to download the files does not work. I think that URL only makes sense on your own computer.
Maybe you could upload the files here:
https://skyline.ms/files.url

[ZeshanAlimq]

Hi Nick,
Thanks for the quick response.
I have uploaded the files with the Zip name Zeshan.

[nickshulman]

Thank you for uploading those files.
Your data does not seem to have any overlapping isolation windows.
The isolation window for every spectrum is [519.76501-528.76501]
You can use ProteoWizard SeeMS.exe to look at your data.

The "unknown exception" that MSConvert is hitting is "integer divide by zero". We should probably fix MSConvert so that it gives a better error message in this case.

[ZeshanAlimq]

Thank you Nick for the help.