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quartz.save(file='./pdf/S02c.pdf', type='pdf') quartz_off_screen 2 + +# Bargraph of TFP-ester labeling gels + +``` r +gel <- read.table('./data/gels/csv/2023-09-07_chickenAlexa488.csv', header = TRUE, sep = ',') + +refDOL <- 5 #ThermoFisher A21200 + +gel$norm <- (gel$Mean/gel$Mean[1])*refDOL + +quartz(width=3.6, height=6.1) + +par(mar=c(6,4,1,0)) +bar <- barplot(gel$norm, ylab='Degree of Labeling (DOL)', las=1, xaxt="n") +labs <- c('Chicken-Alexa488', '1:3', '1:9', '1:15') +text(cex=1, x=c(bar[1]-.55, bar[-1]), y=c(-0.8,rep(-0.4,3)), labs, xpd=TRUE, srt=45) +text(mean(bar[-1]), -1.1, 'Goat-PEG4-TCO\nAZdye488-bis(PEG2-Tz)', xpd=TRUE) +lines(c(bar[2]-.25, bar[4]+.25), rep(-0.75, 2), xpd = TRUE) +``` + +![](supplementary_fig02_files/figure-commonmark/unnamed-chunk-25-1.png) + +Save as PDF: + +``` r +quartz.save(file='./pdf/S02f.pdf', type='pdf') +``` + + quartz_off_screen + 2 diff --git a/supplementary_fig02.qmd b/supplementary_fig02.qmd index 81bb036..e2e44f8 100644 --- a/supplementary_fig02.qmd +++ b/supplementary_fig02.qmd @@ -330,3 +330,28 @@ Lets save the figure as a PDF: ```{r} quartz.save(file='./pdf/S02c.pdf', type='pdf') ``` + +# Bargraph of TFP-ester labeling gels + +```{r} +gel <- read.table('./data/gels/csv/2023-09-07_chickenAlexa488.csv', header = TRUE, sep = ',') + +refDOL <- 5 #ThermoFisher A21200 + +gel$norm <- (gel$Mean/gel$Mean[1])*refDOL + +quartz(width=3.6, height=6.1) + +par(mar=c(6,4,1,0)) +bar <- barplot(gel$norm, ylab='Degree of Labeling (DOL)', las=1, xaxt="n") +labs <- c('Chicken-Alexa488', '1:3', '1:9', '1:15') +text(cex=1, x=c(bar[1]-.55, bar[-1]), y=c(-0.8,rep(-0.4,3)), labs, xpd=TRUE, srt=45) +text(mean(bar[-1]), -1.1, 'Goat-PEG4-TCO\nAZdye488-bis(PEG2-Tz)', xpd=TRUE) +lines(c(bar[2]-.25, bar[4]+.25), rep(-0.75, 2), xpd = TRUE) +``` + +Save as PDF: + +```{r} +quartz.save(file='./pdf/S02f.pdf', type='pdf') +``` diff --git a/supplementary_fig02_files/figure-commonmark/unnamed-chunk-25-1.png 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