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See Version 1 documentation for the old AMRFinder version 1 software
This software and the accompanying database are designed to find acquired antimicrobial resistance genes in bacterial protein or assembled nucleotide sequences as well as known resistance-associated point mutations for several taxa. With AMRFinderPlus we have added select members of additional classes of genes such as virulence factors, biocide, heat, acid, and metal resistance genes.
By default the results returned by AMRFinderPlus will be similar to those returned by the original AMRFinder with some additional data on function. See Running AMRFinderPlus for information on how to get the additional hit types.
This release is incompatible with previous versions of the database. Run amrfinder -u to get the most recent release version of the database before using.
See New in AMRFinderPlus for more information.
AMRFinder requires BLAST and HMMER. We provide instructions using BioConda to install the prerequisites.
Examples
Usage (syntax/options)
Tips and tricks
AMRFinder can be run in multiple modes with protein sequence as input and/or
with DNA sequence as input. To get maximum information it should be run with
both protein and nucleotide. When run with protein sequence it uses both BLASTP
and HMMER to search protein sequences for AMR genes along with a hierarchical
tree of gene families to classify and name novel sequences. With nucleotide
sequences it uses BLASTX translated searches and the hierarchical tree of gene
families. Adding the --organism option enables screening for point mutations
in select organisms and suppresses the reporting of some that are extremely
common in those organisms.
Running AMRFinderPlus
Methods
AMRFinderPlus database
Interpreting results
Many people are using ABRicate with the default "ncbi" database, and it has come to our attention that at least some users are confused that they are getting the same results they would get from AMRFinderPlus. ABRicate uses a subset of the AMRFinderPlus database and different methods to do AMR gene detection, and while we love Torsten and ABRicate, the results are not the same as those you would get by running AMRFinderPlus. To identify AMR genes from assembled sequence we recommend using AMRFinderPlus to get the full value out of the NCBI compiled database, including correct gene symbols, named allele vs. novel allele determination, protein-based search/naming, curated cutoffs, HMM searches, etc.
- Pathogen Detection AMR resources - The Pathogen Detection team at NCBI has a page describing the AMR Resources we provide.
- Pathogen Detection Reference Gene Catalog - Proteins used by AMRFinder in table form with query, filtering, and additional metadata are available in the Reference Gene Catalog. The above information is also included in a tab-delimited file Reference Gene Catalog on FTP.
- Pathogen Detection Isolates Browser - NCBI runs AMRFinderPlus on all microbial genome assemblies in the Pathogen Detection system (over 600,000 isolate assemblies). Gene symbols from AMRFinderPlus runs, along with other metadata for the isolates are visible in the Isolates Browser
- MicroBIGG-E - Detailed AMRFinderPlus results for individual elements, links to sequence, and additional metadata are included in the Pathogen Detection Microbial Browser for Identification of Genetic and Genomic Elements (MicroBIGG-E)
Subscribe to the amrfinder-announce mailing list to get updates when we release new versions of the software or database. This is a low-traffic list.
If you have any questions about or experience problems running AMRFinderPlus, please contact [email protected].
License / Public Domain Notice
See our our AMRFinderPlus paper for more details.
Feldgarden M, Brover V, Gonzalez-Escalona N, Frye JG, Haendiges J, Haft DH, Hoffmann M, Pettengill JB, Prasad AB, Tillman GE, Tyson GH, Klimke W. AMRFinderPlus and the Reference Gene Catalog facilitate examination of the genomic links among antimicrobial resistance, stress response, and virulence. Sci Rep. 2021 Jun 16;11(1):12728. doi: 10.1038/s41598-021-91456-0. PMID: 34135355; PMCID: PMC8208984.
Feldgarden M, Brover V, Haft DH, Prasad AB, Slotta DJ, Tolstoy I, Tyson GH, Zhao S, Hsu CH, McDermott PF, Tadesse DA, Morales C, Simmons M, Tillman G, Wasilenko J, Folster JP, Klimke W. Validating the AMRFinder Tool and Resistance Gene Database by Using Antimicrobial Resistance Genotype-Phenotype Correlations in a Collection of Isolates. Antimicrob Agents Chemother. 2019 Oct 22;63(11):e00483-19. doi: 10.1128/AAC.00483-19. Erratum in: Antimicrob Agents Chemother. 2020 Mar 24;64(4): PMID: 31427293; PMCID: PMC6811410.
- New in AMRFinderPlus
- Documentation for AMRFinder v1 (Depricated)
- Overview
- Install with bioconda (recommended)
- Docker Image
- Install with binary
- Compile from source
- Test your installation
- Usage (syntax/options)
- --organism option
- Examples
- Input file formats
- Output format
- Common errors
- Known issues
- Tips and tricks
- Database updates
- Software upgrades
- Genotypes vs. Phenotypes
- Scope: plus vs. core
- AMRFinderPlus "Method" column
- Element type and Subtype
- Class and Subclass