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RPStblastn subject defline(s) option not taken into account #165

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marieBvr opened this issue Apr 26, 2024 · 3 comments
Open

RPStblastn subject defline(s) option not taken into account #165

marieBvr opened this issue Apr 26, 2024 · 3 comments

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@marieBvr
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Hi,
I use the RPStblastn tool on usegalaxy.eu.

The output I set is an XML BLAST. I would like the ‘query-def’ to be extracted to ‘query-id’ with the advanced option ‘Should the query and subject defline(s) be parsed?’ set to ‘yes’ (as for command line).
This doesn’t work.
When I compare the output with and without the option, I get exactly the same result, as if the option was not taken into account.
Is this normal?

Here is an example of how it should work according to the command line:
Without option:

<Iteration_query-ID>Query_1</Iteration_query-ID>
<Iteration_query-def>NODE_1_length_506_cov_10.687361</Iteration_query-def>

With option:

<Iteration_query-ID>NODE_1_length_506_cov_10.687361</Iteration_query-ID>
<Iteration_query-def>No definition line</Iteration_query-def>

Thanks

@peterjc
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peterjc commented Apr 29, 2024

As I recall, that setting on the search tools (e.g. blastn) depends on how the database was built (i.e. was -parse_seqids used or not).

Are you using your own database, or one of those from the NCBI?

@marieBvr
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I use PFAM-A, which is available in the Galaxy drop-down menu.

@peterjc
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peterjc commented Apr 29, 2024

Cross reference https://help.galaxyproject.org/t/rpstblastn-subject-defline-s/12318 which is probably a better place to discuss this.

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