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This repository has been archived by the owner on Apr 19, 2023. It is now read-only.
Describe the bug
When I am trying to import a seurat rds file using "-profile seurat.rds" the pipelines stops with the error: "Unable to find the required package "SeuratObject". To Reproduce
Steps to reproduce the behavior:
~/nextflow_21.04.3/nextflow -C ../project_name.vsn-pipelines.complete.config \
run vib-singlecell-nf/vsn-pipelines \
-entry single_sample_scenic
See error:
Command error:
WARNING: While bind mounting '/home/user/project/nextflow:/home/user/project/nextflow': destination is already in the mount point list
WARNING: While bind mounting '/home/user/project/nextflow/results/work/79/c26ac4e78b9087536a982da5afdc31:/home/frolova/ageing/nextflow/results/work/79/c26ac4e78b9087536a982da5afdc31': destination is already in the mount point list
WARNING: While bind mounting '/home/user:/home/user': destination is already in the mount point list
Loading required package: SeuratObject
Error in .requirePackage(package) :
unable to find required package ‘SeuratObject’
Calls: @<- ... .extendsForS3 -> extends -> getClassDef -> .requirePackage
In addition: Warning message:
In library(package, lib.loc = lib.loc, character.only = TRUE, logical.return = TRUE, :
there is no package called ‘SeuratObject’
Execution halted
Work dir:
/home/user/project/nextflow/results/work/79/c26ac4e78b9087536a982da5afdc31
Tip: when you have fixed the problem you can continue the execution adding the option `-resume` to the run command line
Cannot append SCENIC loom to SCope loom because the IDs do not match.
-- Check script '/home/user/.nextflow/assets/vib-singlecell-nf/vsn-pipelines/./src/scenic/main.nf' at line: 194 or see '.nextflow.log' file for more details
Expected behavior
Running the whole pipeline, from scanpy to scenic, with the expression matrix imported from the seurat rds file.
Please complete the following information:
OS: Linux 4.18.0-305.12.1.el8_4.x86_64
Nextflow Version: v.21.04.3
vsn-pipelines Version: v.0.27.0
The text was updated successfully, but these errors were encountered:
Describe the bug
When I am trying to import a seurat rds file using "-profile seurat.rds" the pipelines stops with the error: "Unable to find the required package "SeuratObject".
To Reproduce
Steps to reproduce the behavior:
Expected behavior
Running the whole pipeline, from scanpy to scenic, with the expression matrix imported from the seurat rds file.
Please complete the following information:
The text was updated successfully, but these errors were encountered: