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Making use of the Fourier Ring Correlation Implementation by Alex Herbert which is itself 'adapted from the FIRE (Fourier Image REsolution) plugin produced as part of the paper Niewenhuizen, et al (2013). Measuring image resolution in optical nanoscopy. Nature Methods, 10, 557 http://www.nature.com/nmeth/journal/v10/n6/full/nmeth.2448.html

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Fourier Ring Correlation ImageJ Plugin

Making use of the Fourier Ring Correlation Implementation by Alex Herbert which is itself 'adapted from the FIRE (Fourier Image REsolution) plugin produced as part of the paper Niewenhuizen, et al (2013). Measuring image resolution in optical nanoscopy. Nature Methods, 10, 557

http://www.nature.com/nmeth/journal/v10/n6/full/nmeth.2448.html

It is implemented as an Eclipse Plugin, with Maven dependencies for ImageJ/Fiji.

Maintained by Olivier Burri at the BioImaging and Optics Platform (BIOP) http://biop.epfl.ch

Limitations

The following limitations are known and will currently not be adressed. Other packages that are FRC capable should be favored such as NanoJ-SQUIRREL

Known Limitations

  • Scaling due to image size: The Fourier transform extends the image to the next power of two, so be very careful if you are comparing images of different sizes. The recommendation is to use a crop of your data that is a power of two (128, 256, 512, 1024, ...).

Installation

To use this plugin, you can simply activate the PTBIOP update site.

Please refer to the instructions on the Wiki page of the plugin, at http://imagej.net/Fourier_Ring_Correlation_Plugin#Installation

Source Code

Our institution is testing a platform called C4Science, where this file is hosted. Simply use the clone url that is provided above. The simplest way is to use e2git for Eclipse and use Check out Maven project from SCM from Eclipse's Import menu

Todo

Feel free to let us know if more functionality should be added. Perhaps implement the Fourier Shell Correlation for 3D data.

Using the Plugin

You need two images open, the plugin will prompt for the Threshold method to use and will provide the FIRE number as a Results Table and display the FRC curve in a new Plot window.

Batch Mode

You need two folders where each folder has files with the same filenames. The plugin will open each pair of files and run the FRC Calculation on them. It can save the FRC Curve as an image.

License

Copyright (C) 2016 Alex Herbert, Olivier Burri

This program is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.

You should have received a copy of the GNU General Public License along with this program. If not, see http://www.gnu.org/licenses/.

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Making use of the Fourier Ring Correlation Implementation by Alex Herbert which is itself 'adapted from the FIRE (Fourier Image REsolution) plugin produced as part of the paper Niewenhuizen, et al (2013). Measuring image resolution in optical nanoscopy. Nature Methods, 10, 557 http://www.nature.com/nmeth/journal/v10/n6/full/nmeth.2448.html

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