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removing log directives
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LiaOb21 committed Jan 4, 2024
1 parent 7dd13e0 commit dcad458
Showing 1 changed file with 4 additions and 4 deletions.
8 changes: 4 additions & 4 deletions workflow/rules/picard.smk
Original file line number Diff line number Diff line change
Expand Up @@ -21,8 +21,8 @@ rule picard:
"""
mkdir -p results/arima_mapping_pipeline/PAIR_DIR/
mkdir -p results/arima_mapping_pipeline/REP_DIR/
java -Xmx4G -Djava.io.tmpdir=temp/ -jar {PICARD} AddOrReplaceReadGroups INPUT={input.tmp_bam} OUTPUT={output.bam_paired} ID={wildcards.sample} LB={wildcards.sample} SM={wildcards.sample} PL=ILLUMINA PU=none >> {log} 2>&1
java -Xmx30G -XX:-UseGCOverheadLimit -Djava.io.tmpdir=temp/ -jar {PICARD} MarkDuplicates INPUT={output.bam_paired} OUTPUT={output.mark_dup} METRICS_FILE={output.metrics} TMP_DIR=results/arima_mapping_pipeline/TMP_DIR ASSUME_SORTED=TRUE VALIDATION_STRINGENCY=LENIENT REMOVE_DUPLICATES=TRUE >> {log} 2>&1
samtools index {output.mark_dup} >> {log} 2>&1
perl scripts/get_stats.pl {output.mark_dup} > {output.stats} >> {log} 2>&1
java -Xmx4G -Djava.io.tmpdir=temp/ -jar {PICARD} AddOrReplaceReadGroups INPUT={input.tmp_bam} OUTPUT={output.bam_paired} ID={wildcards.sample} LB={wildcards.sample} SM={wildcards.sample} PL=ILLUMINA PU=none
java -Xmx30G -XX:-UseGCOverheadLimit -Djava.io.tmpdir=temp/ -jar {PICARD} MarkDuplicates INPUT={output.bam_paired} OUTPUT={output.mark_dup} METRICS_FILE={output.metrics} TMP_DIR=results/arima_mapping_pipeline/TMP_DIR ASSUME_SORTED=TRUE VALIDATION_STRINGENCY=LENIENT REMOVE_DUPLICATES=TRUE
samtools index {output.mark_dup}
perl scripts/get_stats.pl {output.mark_dup} > {output.stats}
"""

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