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Road Map
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graceful exiting in case star, bowtie2, or trinity crash (lack of memory for example)
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count gene features in the host map bam file for pathway quantification - e.g., innate immunity, hypoxia. this requires a custom GTF file be added to resources/ and a software dependency for the featureCounts program
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open question of how to later analyze orfs_noblastp.fasta (perhaps k-mer composition)
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improve logging and graceful exits: write error to stderr (not stdout), and echo step in the error message (so know where error occurred); determine how to deal with w constituent programs that echo a lot of verbiage to stdout or stderr (do we want this stuff in the main logs, sub-logs, etc.?)
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make a testing framework with sample files, so code can be changed w/o fear of error
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get the machine learning part working: design a panel of RNA (transcriptome) pathogen dilutions (and then calibrate sensitivity etc)
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make SGE mem flags options or configurable via the config file
- "pandora aggregate" will condense the information from individual runs into a single table with a fixed set of features:
- total reads
- number of non-human reads
- summary stats of the contig distribution
- viral load, # viral taxa
- bacterial load, # bacterial taxa
- breakdown of aerobic / facultative / anaerobic bacteria
- breakdown of gram positive / negative bacteria
- recurrent AA motifs from discovery orfs_noblastp.fasta (comma separated list of motif_id's)
- explore correlations between host expression and microbial summary features
- design a 2D calibration panel to promote intuition about sensitivity vs. load vs. genome size etc.
- how to give a probabilistic assignment of taxa present?
- support for SLURM