wip(bcl-pipeline): initial work on bait capture library prep pipeline…

…. Adds pipeline and purposes
sanger · Oct 31, 2023 · 7f9f8c5 · 7f9f8c5
1 parent 0e43eb9
commit 7f9f8c5
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Showing 7 changed files with 112 additions and 19 deletions.
diff --git a/config/exports/exports.yml b/config/exports/exports.yml
@@ -164,3 +164,7 @@ hamilton_lrc_blood_bank_to_lrc_pbmc_bank:
     - wells.transfer_requests_as_target.source_asset
   ancestor_tube_purpose: LRC Blood Vac
   ancestor_purpose: LRC Blood Bank
+rvi_pcr_xp_concentrations_for_custom_pooling:
+  csv: rvi_pcr_xp_concentrations_for_custom_pooling
+  plate_includes: wells.qc_results,wells.aliquots,wells.aliquots.sample
+  ancestor_purpose: RVI Cherrypick
diff --git a/config/pipelines/high_throughput_bcl.yml b/config/pipelines/high_throughput_bcl.yml
@@ -0,0 +1,21 @@
+---
+# Bait Capture Library pipeline configuration
+Bait Capture Library: # Top of the pipeline (Library Prep)
+  pipeline_group: Bait Capture Library
+  filters:
+    request_type_key:
+      - limber_bait_capture_library_prep
+    library_type:
+      - RVI-BCL
+  library_pass:
+    - RVI Cap Lib Pool
+  relationships:
+    RVI Cherrypick: RVI RT
+    RVI RT: RVI cDNA XP
+    RVI cDNA XP: RVI Lib PCR
+    RVI Lib PCR: RVI Lib PCR XP
+    RVI Lib PCR XP: RVI Hyb
+    RVI Hyb: RVI Cap Lib
+    RVI Cap Lib: RVI Cap Lib PCR
+    RVI Cap Lib PCR: RVI Cap Lib PCR XP
+    RVI Cap Lib PCR XP: RVI Cap Lib Pool
diff --git a/config/purposes/rvi.yml b/config/purposes/rvi.yml
@@ -0,0 +1,68 @@
+RVI Cherrypick:
+  :asset_type: plate
+  :stock_plate: false
+  :cherrypickable_target: true
+  :input_plate: false
+  :presenter_class: Presenters::PermissivePresenter
+
+RVI RT:
+  :asset_type: plate
+  :presenter_class: Presenters::PermissivePresenter
+
+RVI cDNA XP:
+  :asset_type: plate
+  :presenter_class: Presenters::PermissivePresenter
+
+RVI Lib PCR:
+  :asset_type: plate
+  :presenter_class: Presenters::PcrPresenter
+  :creator_class: LabwareCreators::TaggedPlate
+  # TODO: this is the full list of templates copied from WGS/ICS and should be reduced
+  :tag_layout_templates:
+    - Illumina pipeline tagging
+    - 'Sanger_168tags - 10 mer tags in columns ignoring pools (first oligo: ATCACGTT)'
+    - hWGS and RNAseq UDI tag layout v3
+    - pWGS UDI tag layout v2 A
+    - pWGS UDI tag layout v2 B
+    - pWGS UDI tag layout v2 C
+    - pWGS UDI tag layout v2 D
+    - TS_pWGSA_UDI96
+    - TS_pWGSB_UDI96
+    - TS_pWGSC_UDI96
+    - TS_pWGSC_UDI_tag60_61_swap
+    - TS_pWGSD_UDI96
+    - TSRNAhWGS_UDI96
+    - TS_pWGSA_UDI96v2
+    - TSISC_UDI96
+
+RVI Lib PCR XP:
+  :asset_type: plate
+  :default_printer_type: :plate_b
+  :label_template: plate_xp
+  :file_links:
+    - name: 'Download Concentration (ng/ul) CSV for Custom Pooling'
+      id: rvi_pcr_xp_concentrations_for_custom_pooling
+
+RVI Hyb:
+  :asset_type: plate
+  :default_printer_type: :plate_b
+  :creator_class: LabwareCreators::BaitedPlate
+  :csv_template: 'show_extended'
+
+RVI Cap Lib:
+  :asset_type: plate
+  :default_printer_type: :plate_b
+
+RVI Cap Lib PCR:
+  :asset_type: plate
+  :default_printer_type: :plate_b
+
+RVI Cap Lib PCR XP:
+  :asset_type: plate
+  :default_printer_type: :plate_b
+
+RVI Cap Lib Pool:
+  :asset_type: plate
+  :default_printer_type: :plate_b
+  :creator_class: LabwareCreators::PlateWithTemplate
+  :transfer_template: 'Pool wells based on submission'
diff --git a/docs/creators.md b/docs/creators.md
@@ -26,8 +26,8 @@ Labware creators are responsible for creating new labware from a parent labware.
 
 {include:LabwareCreators::StampedPlate}
 
-  Used directly in 100 purposes:
-  CLCM DNA End Prep, CLCM DNA Lib PCR XP, CLCM RNA End Prep, CLCM RNA Lib PCR XP, CLCM RT PreAmp, GBS Stock, GBS-96 Stock, GnT MDA Norm, GnT Pico End Prep, GnT Pico-XP, GnT scDNA, GnT Stock, LB Cap Lib, LB Cap Lib PCR, LB Cap Lib PCR-XP, LB cDNA, LB cDNA XP, LB Cherrypick, LB End Prep, LB Lib PCR-XP, LB Post Shear, LB Shear, LBB Cherrypick, LBB Enriched BCR, LBB Enriched BCR HT, LBB Enriched TCR, LBB Enriched TCR HT, LBB Lib-XP, LBB Ligation, LBC 3pV3 GEX Frag 2XP, LBC 3pV3 GEX PCR 2XP, LBC 5p GEX Frag 2XP, LBC 5p GEX PCR 2XP, LBC BCR Enrich1 2XSPRI, LBC BCR Enrich2 2XSPRI, LBC BCR Post PCR, LBC Stock, LBC TCR Enrich1 2XSPRI, LBC TCR Enrich2 2XSPRI, LBC TCR Post PCR, LBR Cherrypick, LBR Frag, LBR Frag cDNA, LBR Globin, LBR Globin DNase, LBR mRNA Cap, LBR Ribo DNase, LBR RiboGlobin DNase, LCA 10X cDNA, LCA PBMC, LCA PBMC Bank, LCMB Cherrypick, LCMB End Prep, LCMB Lib PCR-XP, LDS AL Lib, LDS Cherrypick, LDS Lib PCR XP, LDS Stock, LDS Stock XP, LHR End Prep, LHR PCR 1, LHR PCR 2, LHR RT, LHR-384 AL Lib, LHR-384 End Prep, LHR-384 PCR 1, LHR-384 PCR 2, LHR-384 RT, LHR-384 XP, LSW-96 Stock, LTHR PCR 1, LTHR PCR 2, LTHR RT-S, LTHR-384 PCR 1, LTHR-384 PCR 2, LTN AL Lib, LTN Cherrypick, LTN Lib PCR XP, LTN Post Shear, LTN Shear, LTN Stock, LTN Stock XP, PF Cherrypicked, PF End Prep, PF Lib XP, PF Lib XP2, PF Post Shear, PF Post Shear XP, PF Shear, PF-384 End Prep, PF-384 Lib XP2, pWGS-384 AL Lib, pWGS-384 End Prep, scRNA cDNA-XP, scRNA End Prep, scRNA Stock, scRNA-384 cDNA-XP, scRNA-384 End Prep, scRNA-384 Stock, and Tag Plate - 384
+  Used directly in 107 purposes:
+  CLCM DNA End Prep, CLCM DNA Lib PCR XP, CLCM RNA End Prep, CLCM RNA Lib PCR XP, CLCM RT PreAmp, GBS Stock, GBS-96 Stock, GnT MDA Norm, GnT Pico End Prep, GnT Pico-XP, GnT scDNA, GnT Stock, LB Cap Lib, LB Cap Lib PCR, LB Cap Lib PCR-XP, LB cDNA, LB cDNA XP, LB Cherrypick, LB End Prep, LB Lib PCR-XP, LB Post Shear, LB Shear, LBB Cherrypick, LBB Enriched BCR, LBB Enriched BCR HT, LBB Enriched TCR, LBB Enriched TCR HT, LBB Lib-XP, LBB Ligation, LBC 3pV3 GEX Frag 2XP, LBC 3pV3 GEX PCR 2XP, LBC 5p GEX Frag 2XP, LBC 5p GEX PCR 2XP, LBC BCR Enrich1 2XSPRI, LBC BCR Enrich2 2XSPRI, LBC BCR Post PCR, LBC Stock, LBC TCR Enrich1 2XSPRI, LBC TCR Enrich2 2XSPRI, LBC TCR Post PCR, LBR Cherrypick, LBR Frag, LBR Frag cDNA, LBR Globin, LBR Globin DNase, LBR mRNA Cap, LBR Ribo DNase, LBR RiboGlobin DNase, LCA 10X cDNA, LCA PBMC, LCA PBMC Bank, LCMB Cherrypick, LCMB End Prep, LCMB Lib PCR-XP, LDS AL Lib, LDS Cherrypick, LDS Lib PCR XP, LDS Stock, LDS Stock XP, LHR End Prep, LHR PCR 1, LHR PCR 2, LHR RT, LHR-384 AL Lib, LHR-384 End Prep, LHR-384 PCR 1, LHR-384 PCR 2, LHR-384 RT, LHR-384 XP, LSW-96 Stock, LTHR PCR 1, LTHR PCR 2, LTHR RT-S, LTHR-384 PCR 1, LTHR-384 PCR 2, LTN AL Lib, LTN Cherrypick, LTN Lib PCR XP, LTN Post Shear, LTN Shear, LTN Stock, LTN Stock XP, PF Cherrypicked, PF End Prep, PF Lib XP, PF Lib XP2, PF Post Shear, PF Post Shear XP, PF Shear, PF-384 End Prep, PF-384 Lib XP2, pWGS-384 AL Lib, pWGS-384 End Prep, RVI Cap Lib, RVI Cap Lib PCR, RVI Cap Lib PCR XP, RVI cDNA XP, RVI Cherrypick, RVI Lib PCR XP, RVI RT, scRNA cDNA-XP, scRNA End Prep, scRNA Stock, scRNA-384 cDNA-XP, scRNA-384 End Prep, scRNA-384 Stock, and Tag Plate - 384
 
 {LabwareCreators::StampedPlate View class documentation}
 
@@ -36,8 +36,8 @@ Labware creators are responsible for creating new labware from a parent labware.
 
 {include:LabwareCreators::BaitedPlate}
 
-  Used directly in 1 purposes:
-  LB Hyb
+  Used directly in 2 purposes:
+  LB Hyb and RVI Hyb
 
 {LabwareCreators::BaitedPlate View class documentation}
 
@@ -321,8 +321,8 @@ Labware creators are responsible for creating new labware from a parent labware.
 
 {include:LabwareCreators::PlateWithTemplate}
 
-  Used directly in 1 purposes:
-  LB Cap Lib Pool
+  Used directly in 2 purposes:
+  LB Cap Lib Pool and RVI Cap Lib Pool
 
 {LabwareCreators::PlateWithTemplate View class documentation}
 
@@ -361,8 +361,8 @@ Labware creators are responsible for creating new labware from a parent labware.
 
 {include:LabwareCreators::TaggedPlate}
 
-  Used directly in 20 purposes:
-  CLCM DNA Lib PCR, CLCM RNA Lib PCR, GBS PCR2, GnT Pico Lib PCR, LB Lib PCR, LBSN-384 PCR 2, LCMB Lib PCR, LDS Lib PCR, LHR Lib PCR, LHR-384 Lib PCR, LTHR Lib PCR 1, LTHR Lib PCR 2, LTHR-384 Lib PCR 1, LTHR-384 Lib PCR 2, LTN Lib PCR, PF Lib, PF-384 Lib, pWGS-384 Lib PCR, scRNA Lib PCR, and scRNA-384 Lib PCR
+  Used directly in 21 purposes:
+  CLCM DNA Lib PCR, CLCM RNA Lib PCR, GBS PCR2, GnT Pico Lib PCR, LB Lib PCR, LBSN-384 PCR 2, LCMB Lib PCR, LDS Lib PCR, LHR Lib PCR, LHR-384 Lib PCR, LTHR Lib PCR 1, LTHR Lib PCR 2, LTHR-384 Lib PCR 1, LTHR-384 Lib PCR 2, LTN Lib PCR, PF Lib, PF-384 Lib, pWGS-384 Lib PCR, RVI Lib PCR, scRNA Lib PCR, and scRNA-384 Lib PCR
 
 {LabwareCreators::TaggedPlate View class documentation}
 

diff --git a/docs/presenters.md b/docs/presenters.md
@@ -88,8 +88,8 @@ LDS AL Lib Dil and LTN AL Lib Dil
 
 {include:Presenters::StandardPresenter}
 
-Used directly in 92 purposes:
-CLCM DNA End Prep, CLCM DNA Lib PCR XP, CLCM Lysate DNA, CLCM Lysate RNA, CLCM RNA End Prep, CLCM RNA Lib PCR XP, CLCM RT PreAmp, CLCM Stock, GnT Pico End Prep, GnT Pico-XP, GnT scDNA, LB Cap Lib, LB Cap Lib PCR, LB Cap Lib PCR-XP, LB Cap Lib Pool, LB End Prep, LB Hyb, LB Lib PCR-XP, LB Lib PrePool, LB Post Shear, LB Shear, LBB Enriched BCR, LBB Enriched BCR HT, LBB Enriched TCR, LBB Enriched TCR HT, LBB Lib-XP, LBB Ligation, LBC 3pV3 GEX Frag 2XP, LBC 5p GEX Frag 2XP, LBC Aggregate, LBC BCR Dil 1, LBC BCR Dil 2, LBC BCR Enrich1 2XSPRI, LBC BCR Enrich2 2XSPRI, LBC BCR Post PCR, LBC Cherrypick, LBC TCR Dil 1, LBC TCR Dil 2, LBC TCR Enrich1 2XSPRI, LBC TCR Enrich2 2XSPRI, LBC TCR Post PCR, LBR Frag cDNA, LBR Globin, LCA 10X cDNA, LCA Blood Array, LCA Blood Bank, LCA PBMC, LCA PBMC Bank, LCA PBMC Pools, LCMB End Prep, LCMB Lib PCR-XP, LDS AL Lib, LDS Lib PCR XP, LDS Stock XP, LHR End Prep, LHR PCR 1, LHR PCR 2, LHR XP, LHR-384 AL Lib, LHR-384 cDNA, LHR-384 PCR 1, LHR-384 PCR 2, LHR-384 XP, LRC Blood Bank, LRC PBMC Bank, LSW-96 Stock, LTHR Lib PCR pool, LTHR PCR 1, LTHR PCR 2, LTHR RT-S, LTHR-384 Lib PCR pool, LTHR-384 PCR 1, LTHR-384 PCR 2, LTHR-384 RT-Q, LTN AL Lib, LTN Lib PCR XP, LTN Post Shear, LTN Shear, LTN Stock XP, PF End Prep, PF Lib XP, PF Lib XP2, PF Post Shear, PF Post Shear XP, PF Shear, PF-384 End Prep, PF-384 Lib XP2, PF-384 Post Shear XP, pWGS-384 AL Lib, pWGS-384 Post Shear XP, scRNA cDNA-XP, and scRNA End Prep
+Used directly in 98 purposes:
+CLCM DNA End Prep, CLCM DNA Lib PCR XP, CLCM Lysate DNA, CLCM Lysate RNA, CLCM RNA End Prep, CLCM RNA Lib PCR XP, CLCM RT PreAmp, CLCM Stock, GnT Pico End Prep, GnT Pico-XP, GnT scDNA, LB Cap Lib, LB Cap Lib PCR, LB Cap Lib PCR-XP, LB Cap Lib Pool, LB End Prep, LB Hyb, LB Lib PCR-XP, LB Lib PrePool, LB Post Shear, LB Shear, LBB Enriched BCR, LBB Enriched BCR HT, LBB Enriched TCR, LBB Enriched TCR HT, LBB Lib-XP, LBB Ligation, LBC 3pV3 GEX Frag 2XP, LBC 5p GEX Frag 2XP, LBC Aggregate, LBC BCR Dil 1, LBC BCR Dil 2, LBC BCR Enrich1 2XSPRI, LBC BCR Enrich2 2XSPRI, LBC BCR Post PCR, LBC Cherrypick, LBC TCR Dil 1, LBC TCR Dil 2, LBC TCR Enrich1 2XSPRI, LBC TCR Enrich2 2XSPRI, LBC TCR Post PCR, LBR Frag cDNA, LBR Globin, LCA 10X cDNA, LCA Blood Array, LCA Blood Bank, LCA PBMC, LCA PBMC Bank, LCA PBMC Pools, LCMB End Prep, LCMB Lib PCR-XP, LDS AL Lib, LDS Lib PCR XP, LDS Stock XP, LHR End Prep, LHR PCR 1, LHR PCR 2, LHR XP, LHR-384 AL Lib, LHR-384 cDNA, LHR-384 PCR 1, LHR-384 PCR 2, LHR-384 XP, LRC Blood Bank, LRC PBMC Bank, LSW-96 Stock, LTHR Lib PCR pool, LTHR PCR 1, LTHR PCR 2, LTHR RT-S, LTHR-384 Lib PCR pool, LTHR-384 PCR 1, LTHR-384 PCR 2, LTHR-384 RT-Q, LTN AL Lib, LTN Lib PCR XP, LTN Post Shear, LTN Shear, LTN Stock XP, PF End Prep, PF Lib XP, PF Lib XP2, PF Post Shear, PF Post Shear XP, PF Shear, PF-384 End Prep, PF-384 Lib XP2, PF-384 Post Shear XP, pWGS-384 AL Lib, pWGS-384 Post Shear XP, RVI Cap Lib, RVI Cap Lib PCR, RVI Cap Lib PCR XP, RVI Cap Lib Pool, RVI Hyb, RVI Lib PCR XP, scRNA cDNA-XP, and scRNA End Prep
 
 {Presenters::StandardPresenter View class documentation}
 
@@ -98,8 +98,8 @@ CLCM DNA End Prep, CLCM DNA Lib PCR XP, CLCM Lysate DNA, CLCM Lysate RNA, CLCM R
 
 {include:Presenters::PcrPresenter}
 
-Used directly in 19 purposes:
-CLCM DNA Lib PCR, CLCM RNA Lib PCR, GnT Pico Lib PCR, LB Lib PCR, LBB Chromium Tagged, LBB Lib PCR-XP, LBB Ligation Tagged, LBC 3pV3 GEX LigXP, LBC 3pV3 GEX PCR 2XP, LBC 5p GEX LigXP, LBC 5p GEX PCR 2XP, LBC BCR Post Lig 1XSPRI, LBC TCR Post Lig 1XSPRI, LCA Connect PCRXP, LCMB Lib PCR, LDS Lib PCR, LHR Lib PCR, LTN Lib PCR, and scRNA Lib PCR
+Used directly in 20 purposes:
+CLCM DNA Lib PCR, CLCM RNA Lib PCR, GnT Pico Lib PCR, LB Lib PCR, LBB Chromium Tagged, LBB Lib PCR-XP, LBB Ligation Tagged, LBC 3pV3 GEX LigXP, LBC 3pV3 GEX PCR 2XP, LBC 5p GEX LigXP, LBC 5p GEX PCR 2XP, LBC BCR Post Lig 1XSPRI, LBC TCR Post Lig 1XSPRI, LCA Connect PCRXP, LCMB Lib PCR, LDS Lib PCR, LHR Lib PCR, LTN Lib PCR, RVI Lib PCR, and scRNA Lib PCR
 
 {Presenters::PcrPresenter View class documentation}
 
@@ -108,8 +108,8 @@ CLCM DNA Lib PCR, CLCM RNA Lib PCR, GnT Pico Lib PCR, LB Lib PCR, LBB Chromium T
 
 {include:Presenters::PermissivePresenter}
 
-Used directly in 10 purposes:
-LB cDNA, LB cDNA XP, LBR Frag, LBR Globin DNase, LBR mRNA Cap, LBR Ribo DNase, LBR RiboGlobin DNase, LHR-384 End Prep, PF Lib, and PF-384 Lib
+Used directly in 13 purposes:
+LB cDNA, LB cDNA XP, LBR Frag, LBR Globin DNase, LBR mRNA Cap, LBR Ribo DNase, LBR RiboGlobin DNase, LHR-384 End Prep, PF Lib, PF-384 Lib, RVI cDNA XP, RVI Cherrypick, and RVI RT
 
 {Presenters::PermissivePresenter View class documentation}
 

diff --git a/docs/state_changers.md b/docs/state_changers.md
@@ -18,8 +18,8 @@ manual transfer.
 
 {include:StateChangers::DefaultStateChanger}
 
-  Used directly in 165 purposes:
-  CLCM DNA End Prep, CLCM DNA Lib PCR, CLCM DNA Lib PCR XP, CLCM Lysate DNA, CLCM Lysate RNA, CLCM RNA End Prep, CLCM RNA Lib PCR, CLCM RNA Lib PCR XP, CLCM RT PreAmp, CLCM Stock, GBS PCR1, GBS PCR2, GBS Stock, GBS-96 Stock, GnT MDA Norm, GnT Pico End Prep, GnT Pico Lib PCR, GnT Pico-XP, GnT scDNA, GnT Stock, Heron Lysed Tube Rack, LB Cap Lib, LB Cap Lib PCR, LB Cap Lib PCR-XP, LB Cap Lib Pool, LB cDNA, LB cDNA XP, LB Cherrypick, LB End Prep, LB Hyb, LB Lib PCR, LB Lib PCR-XP, LB Lib PrePool, LB Post Shear, LB Shear, LBB Cherrypick, LBB Chromium Tagged, LBB Enriched BCR, LBB Enriched BCR HT, LBB Enriched TCR, LBB Enriched TCR HT, LBB Lib PCR-XP, LBB Lib-XP, LBB Ligation, LBB Ligation Tagged, LBC 3pV3 GEX Dil, LBC 3pV3 GEX Frag 2XP, LBC 3pV3 GEX LigXP, LBC 3pV3 GEX PCR 2XP, LBC 5p GEX Dil, LBC 5p GEX Frag 2XP, LBC 5p GEX LigXP, LBC 5p GEX PCR 2XP, LBC Aggregate, LBC BCR Dil 1, LBC BCR Dil 2, LBC BCR Enrich1 2XSPRI, LBC BCR Enrich2 2XSPRI, LBC BCR Post Lig 1XSPRI, LBC BCR Post PCR, LBC Stock, LBC TCR Dil 1, LBC TCR Dil 2, LBC TCR Enrich1 2XSPRI, LBC TCR Enrich2 2XSPRI, LBC TCR Post Lig 1XSPRI, LBC TCR Post PCR, LBR Cherrypick, LBR Frag, LBR Frag cDNA, LBR Globin, LBR Globin DNase, LBR mRNA Cap, LBR Ribo DNase, LBR RiboGlobin DNase, LBSN-384 PCR 1, LBSN-384 PCR 2, LCA 10X cDNA, LCA Blood Array, LCA Blood Bank, LCA Connect PCRXP, LCA PBMC, LCA PBMC Bank, LCA PBMC Pools, LCMB Cherrypick, LCMB End Prep, LCMB Lib PCR, LCMB Lib PCR-XP, LDS AL Lib, LDS AL Lib Dil, LDS Cherrypick, LDS Lib PCR, LDS Lib PCR XP, LDS Stock, LDS Stock XP, LDW-96 Stock, LHR End Prep, LHR Lib PCR, LHR PCR 1, LHR PCR 2, LHR RT, LHR XP, LHR-384 AL Lib, LHR-384 cDNA, LHR-384 End Prep, LHR-384 Lib PCR, LHR-384 PCR 1, LHR-384 PCR 2, LHR-384 RT, LHR-384 XP, LILYS-96 Stock, LRC Blood Bank, LRC PBMC Bank, LTHR Cherrypick, LTHR Lib PCR 1, LTHR Lib PCR 2, LTHR Lib PCR pool, LTHR PCR 1, LTHR PCR 2, LTHR RT, LTHR RT-S, LTHR-384 Lib PCR 1, LTHR-384 Lib PCR 2, LTHR-384 Lib PCR pool, LTHR-384 PCR 1, LTHR-384 PCR 2, LTHR-384 RT, LTHR-384 RT-Q, LTN AL Lib, LTN AL Lib Dil, LTN Cherrypick, LTN Lib PCR, LTN Lib PCR XP, LTN Post Shear, LTN Shear, LTN Stock, LTN Stock XP, PF Cherrypicked, PF End Prep, PF Lib, PF Lib Q-XP2, PF Lib XP, PF Lib XP2, PF Post Shear, PF Post Shear XP, PF Shear, PF-384 End Prep, PF-384 Lib, PF-384 Lib XP2, PF-384 Post Shear XP, pWGS-384 AL Lib, pWGS-384 End Prep, pWGS-384 Lib PCR, pWGS-384 Post Shear XP, scRNA cDNA-XP, scRNA End Prep, scRNA Lib PCR, scRNA Stock, scRNA-384 cDNA-XP, scRNA-384 End Prep, scRNA-384 Lib PCR, scRNA-384 Stock, Tag Plate - 384, TR Stock 48, and TR Stock 96
+  Used directly in 175 purposes:
+  CLCM DNA End Prep, CLCM DNA Lib PCR, CLCM DNA Lib PCR XP, CLCM Lysate DNA, CLCM Lysate RNA, CLCM RNA End Prep, CLCM RNA Lib PCR, CLCM RNA Lib PCR XP, CLCM RT PreAmp, CLCM Stock, GBS PCR1, GBS PCR2, GBS Stock, GBS-96 Stock, GnT MDA Norm, GnT Pico End Prep, GnT Pico Lib PCR, GnT Pico-XP, GnT scDNA, GnT Stock, Heron Lysed Tube Rack, LB Cap Lib, LB Cap Lib PCR, LB Cap Lib PCR-XP, LB Cap Lib Pool, LB cDNA, LB cDNA XP, LB Cherrypick, LB End Prep, LB Hyb, LB Lib PCR, LB Lib PCR-XP, LB Lib PrePool, LB Post Shear, LB Shear, LBB Cherrypick, LBB Chromium Tagged, LBB Enriched BCR, LBB Enriched BCR HT, LBB Enriched TCR, LBB Enriched TCR HT, LBB Lib PCR-XP, LBB Lib-XP, LBB Ligation, LBB Ligation Tagged, LBC 3pV3 GEX Dil, LBC 3pV3 GEX Frag 2XP, LBC 3pV3 GEX LigXP, LBC 3pV3 GEX PCR 2XP, LBC 5p GEX Dil, LBC 5p GEX Frag 2XP, LBC 5p GEX LigXP, LBC 5p GEX PCR 2XP, LBC Aggregate, LBC BCR Dil 1, LBC BCR Dil 2, LBC BCR Enrich1 2XSPRI, LBC BCR Enrich2 2XSPRI, LBC BCR Post Lig 1XSPRI, LBC BCR Post PCR, LBC Stock, LBC TCR Dil 1, LBC TCR Dil 2, LBC TCR Enrich1 2XSPRI, LBC TCR Enrich2 2XSPRI, LBC TCR Post Lig 1XSPRI, LBC TCR Post PCR, LBR Cherrypick, LBR Frag, LBR Frag cDNA, LBR Globin, LBR Globin DNase, LBR mRNA Cap, LBR Ribo DNase, LBR RiboGlobin DNase, LBSN-384 PCR 1, LBSN-384 PCR 2, LCA 10X cDNA, LCA Blood Array, LCA Blood Bank, LCA Connect PCRXP, LCA PBMC, LCA PBMC Bank, LCA PBMC Pools, LCMB Cherrypick, LCMB End Prep, LCMB Lib PCR, LCMB Lib PCR-XP, LDS AL Lib, LDS AL Lib Dil, LDS Cherrypick, LDS Lib PCR, LDS Lib PCR XP, LDS Stock, LDS Stock XP, LDW-96 Stock, LHR End Prep, LHR Lib PCR, LHR PCR 1, LHR PCR 2, LHR RT, LHR XP, LHR-384 AL Lib, LHR-384 cDNA, LHR-384 End Prep, LHR-384 Lib PCR, LHR-384 PCR 1, LHR-384 PCR 2, LHR-384 RT, LHR-384 XP, LILYS-96 Stock, LRC Blood Bank, LRC PBMC Bank, LTHR Cherrypick, LTHR Lib PCR 1, LTHR Lib PCR 2, LTHR Lib PCR pool, LTHR PCR 1, LTHR PCR 2, LTHR RT, LTHR RT-S, LTHR-384 Lib PCR 1, LTHR-384 Lib PCR 2, LTHR-384 Lib PCR pool, LTHR-384 PCR 1, LTHR-384 PCR 2, LTHR-384 RT, LTHR-384 RT-Q, LTN AL Lib, LTN AL Lib Dil, LTN Cherrypick, LTN Lib PCR, LTN Lib PCR XP, LTN Post Shear, LTN Shear, LTN Stock, LTN Stock XP, PF Cherrypicked, PF End Prep, PF Lib, PF Lib Q-XP2, PF Lib XP, PF Lib XP2, PF Post Shear, PF Post Shear XP, PF Shear, PF-384 End Prep, PF-384 Lib, PF-384 Lib XP2, PF-384 Post Shear XP, pWGS-384 AL Lib, pWGS-384 End Prep, pWGS-384 Lib PCR, pWGS-384 Post Shear XP, RVI Cap Lib, RVI Cap Lib PCR, RVI Cap Lib PCR XP, RVI Cap Lib Pool, RVI cDNA XP, RVI Cherrypick, RVI Hyb, RVI Lib PCR, RVI Lib PCR XP, RVI RT, scRNA cDNA-XP, scRNA End Prep, scRNA Lib PCR, scRNA Stock, scRNA-384 cDNA-XP, scRNA-384 End Prep, scRNA-384 Lib PCR, scRNA-384 Stock, Tag Plate - 384, TR Stock 48, and TR Stock 96
 
 {StateChangers::DefaultStateChanger View class documentation}
 

diff --git a/spec/controllers/tube_creation_controller_spec.rb b/spec/controllers/tube_creation_controller_spec.rb
@@ -43,7 +43,7 @@
         it 'creates a tube' do
           get :new,
               params: {
-                limber_tube_id: parent_uuid,
+                limber_plate_id: parent_uuid,
                 purpose_uuid: child_purpose_uuid
               },
               session: {
@@ -69,7 +69,7 @@
         it 'creates a tube' do
           post :create,
                params: {
-                 limber_plate_id: parent_uuid,
+                 limber_tube_id: parent_uuid,
                  tube: {
                    purpose_uuid: child_purpose_uuid
                  }
@@ -88,7 +88,7 @@
         it 'creates a tube' do
           post :create,
                params: {
-                 limber_tube_id: parent_uuid,
+                 limber_plate_id: parent_uuid,
                  tube: {
                    purpose_uuid: child_purpose_uuid
                  }