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Chapter 2 Typo #301

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Chapter 2 Typo #301

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2 changes: 1 addition & 1 deletion jupyter-book/introduction/scrna_seq.md
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Expand Up @@ -38,7 +38,7 @@ It is unfortunately not always possible to find such "simple" associations betwe
Although DNA was already first isolated in 1869 by Friedrich Miescher, it took the scientific community more than 100 years to develop high throughput sequencing technologies.
In 1953, Watson, Crick and Franklin discovered the structure of DNA; and in 1965 Robert Holley sequenced the first tRNA.
Seven years later, in 1972, Walter Fiers was the first to sequence a complete gene (the coat protein of bacteriophage MS2) using RNAses to digest the virus RNA, isolate oligonucleotides and finally separate them with electrophoresis and chromatography{cite}`JOU1972`.
In parallel, Friedrich Sanger developed a DNA sequencing method using radiolabeled, partially digested fragments termed "chain termination method", which is more commonly known as "Sanger Sequencing". Although Sanger Sequencing is still used even today, it suffered from several shortcomings, including lack of automation and being time-consuming.
In parallel, Frederick Sanger developed a DNA sequencing method using radiolabeled, partially digested fragments termed "chain termination method", which is more commonly known as "Sanger Sequencing". Although Sanger Sequencing is still used even today, it suffered from several shortcomings, including lack of automation and being time-consuming.
In 1987, Leroy Hood and Michael Hunkapiller developed the ABI 370, an instrument that automates the Sanger Sequencing process. Its most important innovative accomplishment was the automatic labeling of DNA fragments with fluorescent dyes instead of radioactive molecules. This change not only made the method safer to perform, but also allowed for computers to analyze the acquired data{cite}`Hood1987`.

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