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| Original file line number | Diff line number | Diff line change |
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| @@ -0,0 +1,141 @@ | ||
| #!/usr/bin/env bash | ||
| # prepare_lancet_input.sh: Turn a directory of Lancet output into a Tube Map BED file and directory that can be hosted on the web. | ||
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| function usage() { | ||
| echo >&2 "${0}: Prepare a directory of Lancet data ." | ||
| echo >&2 | ||
| echo >&2 "The input directory should have in it:" | ||
| echo >&2 "- variant_calling_regions.with_calls.bed, an extended BED file with biallelic VCF REF in column 6, ALT in column 7, and INFO in column 9 with a TYPE field" | ||
| echo >&2 "- giraffe_alignments/, a directory with, for each VCF record:" | ||
| echo >&2 " - <CONTIG>_<START>_<END>.giraffe.gbz" | ||
| echo >&2 " - normal.<CONTIG>_<START>_<END>.gam" | ||
| echo >&2 " - tumor.<CONTIG>_<START>_<END>.gam" | ||
| echo >&2 "The ranges in the file named will not correspond exactly to those in the BED records; files for a range overlapping and centered on the BED record will be used." | ||
| echo >&2 | ||
| echo >&2 "Usage: ${0} <INPUT_DIR> <OUTPUT_DIR>" | ||
| exit 1 | ||
| } | ||
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| INPUT_DIR="${1}" | ||
| OUTPUT_DIR="${2}" | ||
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| if [[ -z "${OUTPUT_DIR}" || -z "${INPUT_DIR}" || ! -d "${INPUT_DIR}" ]] ; then | ||
| usage | ||
| fi | ||
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| # Make all the paths absolute | ||
| INPUT_DIR="$(realpath "${INPUT_DIR}")" | ||
| OUTPUT_DIR="$(realpath "${OUTPUT_DIR}")" | ||
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| INPUT_BED="${INPUT_DIR}/variant_calling_regions.with_calls.bed" | ||
| INPUT_DATA_DIR="${INPUT_DIR}/giraffe_alignments" | ||
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| if [[ ! -f "${INPUT_BED}" || ! -d "${INPUT_DATA_DIR}" ]] ; then | ||
| usage | ||
| fi | ||
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| SCRIPTS_DIR="$(dirname "$(realpath "${BASH_SOURCE[0]}")")" | ||
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| mkdir -p "${OUTPUT_DIR}" | ||
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| OUTPUT_BED="${OUTPUT_DIR}/index.bed" | ||
| rm -f "${OUTPUT_BED}" | ||
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| # We need to ber in the output directory to use relative paths in the generated BED. | ||
| cd "${OUTPUT_DIR}" | ||
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| while IFS="" read -r INPUT_LINE || [[ -n "${INPUT_LINE}" ]] ; do | ||
| # Read all the lines, even if the newline is missing. See <https://stackoverflow.com/a/1521498> | ||
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| # Parse the BED | ||
| CONTIG="$(echo "${INPUT_LINE}" | cut -f1)" | ||
| # These are 0-based, end-exclusive coordinates | ||
| START_BED_POS="$(echo "${INPUT_LINE}" | cut -f2)" | ||
| END_BED_POS="$(echo "${INPUT_LINE}" | cut -f3)" | ||
| REF_SEQ="$(echo "${INPUT_LINE}" | cut -f6)" | ||
| ALT_SEQ="$(echo "${INPUT_LINE}" | cut -f7)" | ||
| INFO="$(echo "${INPUT_LINE}" | cut -f9)" | ||
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| # Compute 1-based, end-inclusive coordinates | ||
| START_POS=$((START_BED_POS+1)) | ||
| END_POS="${END_BED_POS}" | ||
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| # Fetch out the type (MNP, INS, etc.) | ||
| VARIANT_TYPE="$(echo "${INFO}" | grep -o "TYPE=[A-Z]*" | cut -f2 -d'=')" | ||
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| # Find the best overlapping graph file. | ||
| # These are the 1-based, end-exclusive coordinates from the filenames | ||
| BEST_START="" | ||
| BEST_END="" | ||
| # These are the 0-based, end-exclusive coordinates | ||
| BEST_BED_START="" | ||
| BEST_BED_END="" | ||
| # This is how many more bases are on one side fo the variant than are on the other | ||
| BEST_UNEVENNESS="" | ||
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| CANDIDATES=("${INPUT_DATA_DIR}/${CONTIG}_"*".giraffe.gbz") | ||
| for GRAPH_FILE in "${CANDIDATES[@]}" ; do | ||
| GRAPH_BASENAME="$(basename "${GRAPH_FILE}")" | ||
| # Work out the region this graph covers. Remember to convert to 1-based | ||
| GRAPH_START="$(echo "${GRAPH_BASENAME%.giraffe.gbz}" | cut -f2 -d'_')" | ||
| GRAPH_END="$(echo "${GRAPH_BASENAME%.giraffe.gbz}" | cut -f3 -d'_')" | ||
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| # Convert to 0-based, end-exclusive | ||
| GRAPH_BED_START=$((GRAPH_START-1)) | ||
| GRAPH_BED_END="${GRAPH_END}" | ||
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| BEFORE_BASES=$((START_BED_POS-GRAPH_BED_START)) | ||
| AFTER_BASES=$((GRAPH_BED_END-END_BED_POS)) | ||
| if [[ "${BEFORE_BASES}" -lt 0 || "${AFTER_BASES}" -lt 0 ]] ; then | ||
| # This graph does not overlap the variant we are working on | ||
| continue | ||
| fi | ||
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| # Work out how uneven the centering is | ||
| if [[ "${BEFORE_BASES}" -gt "${AFTER_BASES}" ]] ; then | ||
| UNEVENNESS=$((BEFORE_BASES-AFTER_BASES)) | ||
| else | ||
| UNEVENNESS=$((AFTER_BASES-BEFORE_BASES)) | ||
| fi | ||
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| if [[ -z "${BEST_UNEVENNESS}" || "${BEST_UNEVENNESS}" -gt "${UNEVENNESS}" ]] ; then | ||
| # This is the best graph so far | ||
| BEST_START="${GRAPH_START}" | ||
| BEST_END="${GRAPH_END}" | ||
| BEST_BED_START="${GRAPH_BED_START}" | ||
| BEST_BED_END="${GRAPH_BED_END}" | ||
| BEST_UNEVENNESS="${UNEVENNESS}" | ||
| fi | ||
| done | ||
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| if [[ -z "${BEST_START}" ]] ; then | ||
| echo >&2 "No graph in ${INPUT_DATA_DIR} overlaps the variant ${INPUT_LINE}" | ||
| exit 1 | ||
| fi | ||
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| # Find the best graph again | ||
| INPUT_GRAPH="$(find "${INPUT_DATA_DIR}" -name "${CONTIG}_${BEST_START}_${BEST_END}.giraffe.gbz")" | ||
| INPUT_GRAPH_BASENAME="$(basename "${INPUT_GRAPH}")" | ||
| SLUG="${INPUT_GRAPH_BASENAME%.giraffe.gbz}" | ||
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| INPUT_NORMAL_GAM="${INPUT_DATA_DIR}/normal.${SLUG}.gam" | ||
| INPUT_TUMOR_GAM="${INPUT_DATA_DIR}/tumor.${SLUG}.gam" | ||
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| # Make the chunk directory. Remember to use a 1-based, end-inclusive range and description coordinates. | ||
| "${SCRIPTS_DIR}/prepare_local_chunk.sh" \ | ||
| -x "${INPUT_GRAPH}" \ | ||
| -g "${INPUT_NORMAL_GAM}" \ | ||
| -p '{"mainPalette": "blues", "auxPalette": "blues"}' \ | ||
| -g "${INPUT_TUMOR_GAM}" \ | ||
| -p '{"mainPalette": "reds", "auxPalette": "reds"}' \ | ||
| -r "${CONTIG}:${BEST_START}-${BEST_END}" \ | ||
| -d "Tumor-specific ${REF_SEQ} -> ${ALT_SEQ} ${VARIANT_TYPE} variant at ${CONTIG}:${START_POS}" \ | ||
| -o "${SLUG}" \ | ||
| >>"${OUTPUT_BED}" | ||
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| # We want to hide the path cover paths from Giraffe and only show the paths from the original Lancet GFA | ||
| vg paths --drop-paths --paths-by "path_cover_" -x "${SLUG}/chunk.vg" >"${SLUG}/chunk.vg.new" | ||
| mv "${SLUG}/chunk.vg.new" "${SLUG}/chunk.vg" | ||
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| done <"${INPUT_BED}" |
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